Heterologous Expression of Membrane Proteins

Methods and Protocols
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This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

Mehr Informationen
ReiheMethods in Molecular Biology
ISBN 9781071623671
Sprache Englisch
Ausgabe 3rd ed. 2022
Erscheinungsdatum 01.07.2022
Umfang 467 Seiten
Genre Biologie/Mikrobiologie
Format Hardcover
Verlag Springer US
Herausgegeben von Isabelle Mus-Veteau
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