Gene Isolation and Mapping Protocols

332 Seiten, Taschenbuch

Kurzbeschreibung des Verlags

Gene Mapping Goes from FISH to Surfing the Net.- Linkage Analysis of Genetic Disorders.- Gene Ordering and Localization by Linkage Analysis.- Gene Mapping Using Somatic Cell Hybrids.- Gene Mapping by FISH.- Probe Ordering and Distancing by FISH.- Physical Mapping by Pulsed-Field Gel Electrophoresis.- Construction and Use of YAC Contigs in Disease Regions.- Construction and Use of Cosmid Contigs.- Use of Dinucleotide Polymorphism Analyses in Physical Mapping.- Mapping Expressed Sequence Tags (ESTs) by Multiplexing PCR Reactions from Hybrid Cell Panels and Detecting Fluorescently Labeled Products.- Gene Isolation by Exon Trapping.- Isolation of Coding Sequences from Genomic Regions Using Direct Selection.- Isolation of cDNAs Using the YAC Hybridization Screen Method.- Detection and Isolation of Differentially Expressed Genes by Differential Display.- Chemical Crosslinking Subtraction (CCLS).- Gene Mapping and Isolation.- Software for Genetic Linkage Analysis.- Exon Detection by Similarity Searches.

• Reihe: Methods in Molecular Biology
• Themen: Mathematik und Naturwissenschaften Biologie, Biowissenschaften Zellbiologie (Zytologie)
• ISBN: 9780896033825
• Sprache: Englisch
• Erscheinungsdatum: 19.10.1996
• Größe: 229 x 152 mm
• Verlag: Humana
• Herausgegeben von: Jacqueline Boultwood