Eicosanoid Protocols

312 Seiten, Taschenbuch

Kurzbeschreibung des Verlags

Use of a Retroviral Vector for Expression of a Foreign Gene.- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues.- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization.- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin.- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography.- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry.- Assessment of Cyclooxygenase Protein Expression by Western Blotting.- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells.- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction.- Enzyme immunoassays (EIA) of Eicosanoids.- Transcellular Regulation of Eicosanoid Biosynthesis.- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry.- Epoxyeicosatrienoic Acids.- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts.- Assessment of 5-Lipoxygenase Activity and Cellular Distribution.- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids.- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition.- Novel Eicosanoids.

• Reihe: Methods in Molecular Biology
• Themen: Mathematik und Naturwissenschaften Biologie, Biowissenschaften Biochemie
• ISBN: 9780896036673
• Sprache: Englisch
• Erscheinungsdatum: 25.03.1999
• Größe: 229 x 152 mm
• Verlag: Humana
• Herausgegeben von: Elias A Lianos