Metabolic Flux Analysis in Eukaryotic Cells

Methods and Protocols

370 Seiten, Taschenbuch

Kurzbeschreibung des Verlags

This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols  is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.

• Reihe: Methods in Molecular Biology
• Themen: Mathematik und Naturwissenschaften Biologie, Biowissenschaften Biowissenschaften, allgemein DV-gestützte Biologie/Bioinformatik
• ISBN: 9781071601617
• Sprache: Englisch
• Erscheinungsdatum: 26.08.2021
• Größe: 25.4 x 17.8 cm
• Verlag: Springer US
• Herausgegeben von: Deepak Nagrath