CRISPR-Cas Methods

278 Seiten, Hardcover

Kurzbeschreibung des Verlags

This volume details the fundamentals of the CRISPR-Cas system , and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.  Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

• Reihe: Springer Protocols Handbooks
• Themen: Mathematik und Naturwissenschaften Biologie, Biowissenschaften Biowissenschaften, allgemein Genetik (nicht-medizinisch)
• ISBN: 9781071606155
• Sprache: Englisch
• Erscheinungsdatum: 30.06.2020
• Größe: 25.4 x 17.8 cm
• Verlag: Springer US
• Herausgegeben von: M. Tofazzal Islam, Pankaj K. Bhowmik, Kutubuddin A. Molla