Histone Methyltransferases

Methods and Protocols

495 Seiten, Hardcover

Kurzbeschreibung des Verlags

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro,  generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology  series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols  aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

• Reihe: Methods in Molecular Biology
• Themen: Mathematik und Naturwissenschaften Biologie, Biowissenschaften Chemische Biologie
• ISBN: 9781071624807
• Sprache: Englisch
• Erscheinungsdatum: 24.06.2022
• Größe: 25.4 x 17.8 cm
• Verlag: Springer US
• Herausgegeben von: Raphaël Margueron, Daniel Holoch