Plant Synthetic Promoters

Methods and Protocols

325 Seiten, Hardcover

Kurzbeschreibung des Verlags

This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of
cis
-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the
cis
-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful 
Methods in Molecular Biology
series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. 

Practical and cutting-edge,
Plant Synthetic Promoters: Methods and Protocols 
covers all steps required from the identification of
cis
-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

• Reihe: Methods in Molecular Biology
• ISBN: 9781493963942
• Sprache: Englisch
• Erscheinungsdatum: 25.08.2016
• Genre: Biologie/Botanik
• Verlag: Springer US
• Herausgegeben von: Reinhard Hehl