Purinergic Signaling

Methods and Protocols

360 Seiten, Hardcover

Kurzbeschreibung des Verlags

This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models,  
in silico
modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge,
Purinergic Signaling: Methods and Protocols
will provide asound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.

• Reihe: Methods in Molecular Biology
• ISBN: 9781493997169
• Sprache: Englisch
• Erscheinungsdatum: 24.10.2019
• Genre: Biologie/Mikrobiologie
• Verlag: Springer US
• Herausgegeben von: Pablo Pelegrín