Organelle Proteomics

421 Seiten, Hardcover

Kurzbeschreibung des Verlags

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

• Reihe: Methods in Molecular Biology
• ISBN: 9781588297792
• Sprache: Englisch
• Erscheinungsdatum: 19.02.2008
• Genre: Biologie/Biochemie, Biophysik
• Verlag: Humana Press
• Herausgegeben von: Delphine Pflieger, Jean Rossier